Deskripsi

Himedia Dichloran Glycerol Medium Base 500gr M1129-500G

Himedia Dichloran Glycerol Medium Base 500gr M1129-500G

Dichloran Glycerol Medium Base M1129
Recommended for selective isolation of xerophilic moulds from food and clinical samples.

Composition**
Ingredients Gms / Litre
Peptone 5.000
Dextrose (Glucose) 10.000
Potassium dihydrogen phosphate 1.000
Magnesium sulphate 0.500
Dichloran 0.002
Chloramphenicol 0.100
Agar 15.000
Final pH ( at 25°C) 5.6±0.2
**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 15.8 grams in 500 ml purified / ddistilled water. Heat to boiling to ddissolve the medium completely. Add 110 grams
of glycerol (Analytical Reagent Grade). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to
45-50°C. Mix well and pour into sterile Petri plates.

Principle And Interpretation

DDichloran Glycerol Medium was formulated by Hocking and Pitt (2) and is recommended for isolation and enumeration of
xerophilic moulds from dried and semidried foods. The glycerol at 18% (w/w) lowers the water activity (aw) from 0.999 to
0.95 (1) without causing any problem. This restrictive characteristic makes the medium especially suitable for foods. Peptone
provides carbon, nitrogen, vitamins and minerals. Dextrose (Glucose) is a carbohydrate source.

Phosphate buffers themedium.

Magnesium sulfate provides ddivalent cations and sulfate. dDichloran is an antifungal agent, added to the medium to
reduce colony ddiameters of spreading fungi.This medium can also be used for isolation of fungi from clinical samples.
Chloramphenicol is included to inhibit the growth of bacteria present in environmental and food samples. Inhibition of
growth of bacteria and restriction of spreading of more-rapidly growing moulds aids in the isolation of slow-growing fungi by
preventing their overgrowth by more-rapidly growing species.

Process 40 gm of food sample in a stomacher by adding 200 ml of 0.1% Peptone Water (M028). Shake periodically for 30
minutes with 0.1% Peptone Water for powdered products. DDilute the sample to 1:10 in 0.1% Peptone water and spread on
plate. Count the number of Xerophilic colonies per gram of food. The medium can also be used as general medium for the
isolation of yeasts and moulds from foodstuffs (1).
Intended use

Type of specimen

Clinical samples – skin scrapings, Food samples
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4).
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (5).
After use, contaminated materials must be sterilized by autoclaving before ddiscarding.

Specimen Collection and Handling:

Warning and Precautions
In Vitro ddiagnostic Use. Read the label before opening the container. Wear protective gloves/protective clothing/eye
protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be
referred in individual safety data sheets