Deskripsi

Himedia M121-500G Brilliant Green Bile Broth 2%, Powder

Himedia M121-500G Brilliant Green Bile Broth 2%, Powder

Intended Use:
Recommended for detection and confirmation of coliform bacteria in water, waste water, food, milk and dairy products.

Composition**

Ingredients Gms / Litre
Peptone 10.000
Lactose 10.000
Bile# 20.000
Brilliant green 0.0133
Final pH ( at 25°C) 7.2±0.2
**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 40.01 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Distribute in
fermentation tubes containing inverted Durhams tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Cool to 45-50°C. Mix well and pour into sterile Petri plates. For preparation of double strength it is recommended to heat the
ddissolved broth (80.02 grams per litre) at 100°C for 30 minutes.

Principle And Interpretation

Brilliant Green Bile Broth 2% is one of the most widely used medium for the detection of coliform bacteria in water,
wastewater, foods, and milk and dairy products. This medium is formulated as per APHA (3,9,10) for the presumptive
identification and confirmation of coliform bacteria (7,8). This medium is also recommended by the ISO Committee for
enumeration of coliforms by most probable number technique (4).

Peptone serves as a source of essential nutrients.

Lactose is the fermentable carbohydrate. Bile inhibits gram-positive bacteria
whereas the gram-negative bacteria are inhibited by brilliant green. Production of gas from lactose fermentation is detected
by incorporating inverted Durham’s tube, which indicates the positive evidence of faecal coliform since non faecal coliforms
growing in this medium do not produce gas. Further gas production in EC broth (M127) at 45°C used as a confirmation of
faecal coliform. Gram-positive spore formers may produce gas if the bile or brilliant green inhibition is weakened by reaction
with food material.

During examination of water samples, growth from presumptive positive tubes showing gas in Lactose Broth (M026) or
Lauryl Tryptose Broth (M080) is inoculated in Brilliant Green Bile Broth 2% (M121). Gas formation within 48 ± 2 hours
confirms the presumptive test (3).
# – Equivalent to Oxgall

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